近日，我院劳动卫生与环境卫生研究团队在研究纳米氧化铁细胞氧化还原酶系稳态方面研究取得新进展，为吸入钢铁粉尘等含铁颗粒物的毒性损伤预防提供科学依据。相关研究成果以“Intracellular GSH/GST antioxidants system change as an earlier biomarker for toxicity evaluation of iron oxide nanoparticles”为题，发表在期刊NanoImpact（IF=5.316）（DOI 10.1016/j.impact.2021.100338）上。
Recently, the research team of occupational and environmental health of our school has made new progress in the study of early biomarkers of the toxic effect of iron oxide nanoparticles (mainly Fe3O4 particles) on human body. The related research results were published in the journal NanoImpact (IF=5.316) (DOI 10.1016/j.impact.2021.100338) under the title of "Intracellular GSH/GST antioxidants system change as an earlier biomarker for toxicity evaluation of iron oxide nanoparticles".
Iron oxide nanoparticles stemmed from traffic exhaust, steel manufacturing, or welding as a potential environmental pollution can lead to adverse respiratory outcomes and aggravate the risk of chronic health conditions via persistent oxidative stress. Glutathione (GSH) and glutathione-S-transferases (GSTs) are two frontlines of cellular defense against both acute and chronic toxicity of xenobiotics-induced oxidative stress. The contribution of GSH and GST enzymes to signaling pathways and the regulation of GSH homeostasis play a central role in the detoxification of numerous environmental toxins and impurities.Based on this, directed by Prof. Jinglong Tang, the research team conducted two kinds of acute exposure experiments of iron oxide (Fe2O3 and Fe3O4) nanoparticles in cells and in vivo, and evaluated the GSH levels and GST activity after exposure to assess the role of GSH/GST antioxidants system in the body's resistance caused by iron oxide nanoparticles.
It was found that Fe3O4 nanoparticles at lower concentrations (≤100 μg/ml) seem to be more toxic to the human bronchial epithelial cells as their consumption of GSH and decrease of GST activity. The catalysis activity of Fe3O4 nanoparticles per se may contribute to the intracellular GSH consumption along with inhibition of glutathione-S-transferase class M and P (GSTM1 and GSTP1) active site and expression decrease of GSTM1 and GSTP1. Accordingly, the GSH consumption and decrease in GST activity directed to the further lipid peroxidation regarded as an earlier marker for toxicity evaluation of iron oxide nanoparticles, and relevant intervention may be effective for prevention of respiratory exposure induced damage from iron oxide nanoparticles. Prof. Jinglong Tang and Mingliang Zhang were co-supervised the study. Graduate students Wanjun Zhang, Jinling Gao (Now at Department of Infection Management Service, Dushu Lake Hospital Affiliated of Soochow University) were the co-first authors. The above research was supported by the National Science Foundation of China (81872651, 92043202, and 91943301) and the Ministry of Science and Technology of China (National Key Research and Development Project 2017YFC1600200).